mhc iix exclusion Search Results


mhc iix exclusion  (Developmental Studies Hybridoma Bank)
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Developmental Studies Hybridoma Bank mhc iix exclusion
Lmod2 WT + cGFP and KO + cGFP-Lmod2 skeletal muscle cross sections were stained with antibodies against different <t>MHC</t> isoforms. Representative images of EDL ( A ) or soleus ( B ) MHC fiber type staining. Top: MHC type I (blue), MHC type IIB (green), and laminin (red). Middle: MHC type IIA (green) and laminin (red). Bottom: MHC type <t>IIX</t> (blue) and laminin (red). Quantification of the number of MHC type I–, IIA–, IIB–, or IIX–positive fibers in EDL ( C ) or soleus ( E ) cross-sections shown as percent of total fibers. Minimum Feret diameter of MHC type I–, IIA–, IIB–, or IIX–positive fibers in EDL ( D ) or soleus ( F ) cross sections. Scale bars, 100 μm. All values are means ± SD; P < 0.05 was considered significant, unpaired t test; * P < 0.05, ** P < 0.01; n = 4 mice per genotype.
Mhc Iix Exclusion, supplied by Developmental Studies Hybridoma Bank, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Lmod2 WT + cGFP and KO + cGFP-Lmod2 skeletal muscle cross sections were stained with antibodies against different MHC isoforms. Representative images of EDL ( A ) or soleus ( B ) MHC fiber type staining. Top: MHC type I (blue), MHC type IIB (green), and laminin (red). Middle: MHC type IIA (green) and laminin (red). Bottom: MHC type IIX (blue) and laminin (red). Quantification of the number of MHC type I–, IIA–, IIB–, or IIX–positive fibers in EDL ( C ) or soleus ( E ) cross-sections shown as percent of total fibers. Minimum Feret diameter of MHC type I–, IIA–, IIB–, or IIX–positive fibers in EDL ( D ) or soleus ( F ) cross sections. Scale bars, 100 μm. All values are means ± SD; P < 0.05 was considered significant, unpaired t test; * P < 0.05, ** P < 0.01; n = 4 mice per genotype.

Journal: Science Advances

Article Title: Lmod2 is necessary for effective skeletal muscle contraction

doi: 10.1126/sciadv.adk1890

Figure Lengend Snippet: Lmod2 WT + cGFP and KO + cGFP-Lmod2 skeletal muscle cross sections were stained with antibodies against different MHC isoforms. Representative images of EDL ( A ) or soleus ( B ) MHC fiber type staining. Top: MHC type I (blue), MHC type IIB (green), and laminin (red). Middle: MHC type IIA (green) and laminin (red). Bottom: MHC type IIX (blue) and laminin (red). Quantification of the number of MHC type I–, IIA–, IIB–, or IIX–positive fibers in EDL ( C ) or soleus ( E ) cross-sections shown as percent of total fibers. Minimum Feret diameter of MHC type I–, IIA–, IIB–, or IIX–positive fibers in EDL ( D ) or soleus ( F ) cross sections. Scale bars, 100 μm. All values are means ± SD; P < 0.05 was considered significant, unpaired t test; * P < 0.05, ** P < 0.01; n = 4 mice per genotype.

Article Snippet: The following primary antibodies were used: MHC I [3.5 mg/ml; BA-F8 IgG2b, Developmental Studies Hybridoma Bank (DSHB)], MHC IIB (9 mg/ml; BF-F3 IgM, DSHB), MHC IIA (3.5 mg/ml; SC-71 IgG1, DSHB), MHC IIX exclusion (3.5 mg/ml; BF-35 IgG1, DSHB), pan-myosin (9 mg/ml; A4-1025 IgG2a, DSHB), and rabbit polyclonal laminin (1:400; L9393, Sigma-Aldrich).

Techniques: Staining